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. 2019 Jun 24;8:e48165. doi: 10.7554/eLife.48165

Figure 7. Mutations in quinone-binding pocket of Complex II subunit MEV-1 affect ability to survive extended KCN treatment.

Figure 7.

(a) Positions of mutations that alter wact-11 sensitivity. MEV-1 structures show either the wild-type residues or mutations that alter wact-11 binding. The structures shown are C. elegans sequences (green) threaded onto the Ascaris suum crystal (pink). RQ is shown in yellow with the critical amine group in blue — note that this is RQ2 and not RQ9, hence the shortened prenyl tail. Note that G71E alters a residue that lies right over the quinone ring of RQ whereas C78Y is further from the ring. (b) Effect of quinone-pocket mutations on ability to survive extended KCN exposure. L1 worms containing either wild-type, G71E or C78Y mutant mev-1 alleles were exposed to 200 µM KCN for 15 hr. KCN was then diluted 6x and the movement of worms measured across a 3 hr time course. Curves show means of 3 biological replicates with three technical replicates in each; error bars are standard error.