Table 2. Transport properties of WT and mutated Slc26a9^T.

		WT*	Q88A	F92A	T127A	F128A	L391A	S392A	N441A
SCN–	Erev (mV)† Px/PCl Gx/GCl	50.5 7.5 0.43	58.4 10.6 0.55	37.8 4.5 0.44	59.3 10.7 0.80	68.7 14.9 1.46	61.5 12.0 1.10	71.5 18.0 1.39	52.9 8.2 0.57
I–	Erev (mV) Px/PCl Gx/GCl	26.6 2.9 0.23	16.3 1.9 0.14	14.1 1.8 0.25	9.5 1.5 0.17	31.0 3.5 0.53	23.5 2.6 0.54	53.2 8.4 1.31	29.1 3.2 0.35
Br–	Erev (mV) Px/PCl Gx/GCl	21.3 2.4 0.60	3.7 1.2 0.32	5.1 1.2 0.72	7.6 1.4 0.40	24.6 2.7 1.13	16.1 1.9 1.11	40.7 5.2 1.78	20.0 2.2 0.70
NO3–	Erev (mV) Px/PCl Gx/GCl	9.4 1.5 0.49	32.6 3.7 1.20	11.2 1.6 0.52	29.0 3.2 1.46	28.5 3.1 1.04	20.7 2.3 0.70	39.1 4.8 1.31	16.9 2.0 0.77
Cl–	Erev (mV) Px/PCl Gx/GCl	−0.1 1 1	−1.4 1 1	−1.5 1 1	−0.5 1 1	0 1 1	−0.7 1 1	0.3 1 1	−0.3 1 1
F–	Erev (mV) Px/PCl Gx/GCl	−81.1 .05 nd¶	−64.2 .08 nd	−81.0 .04 nd	−74.5 .07 nd	−67.6 .07 nd	−81.0 .05 nd	−58.8 0.11 nd	−86.7 0.03 nd
HCO3–	Erev (mV) Px/PCl Gx/GCl	−81.7 0.05 nd	−54.4 0.12 nd	−69.5 0.07 nd	−84.7 0.04 nd	−47.3 0.16 nd	−93.1 0.03 nd	−57.4 0.10 nd	−76.0 0.05 nd
SO42–	Erev (mV) Px/PCl Gx/GCl	−96.9 0.01 nd	−102.2 0.01 nd	−78.0 0.01 nd	−104.5 0 nd	−70.5 0.02 nd	−93.6 0.01 nd	−71.2 0.02 nd	−82.4 0.01 nd
	E30mM Cl‡	−37.7	−39.3	−38.5	−39.8	−42.1	−37.9	−39.0	−38.3
	Km,Cl (mM) §	29.5	2.3	19.2	4.7	18.6	44.0	54.1	31.3
	Km,SCN (mM) §	0.5	0.2	0.8	1.6	5.2	29.4	3.8	0.7
	VSCN/VCl §	0.44	0.63	0.44	0.74	1.08	1.08	0.83	0.68

^*WT refers to non-mutated Slc26a9^T.

^†E_rev values, P_x/P_Cl, and G_x/G_Cl were derived from bi-ionic data in Figure 1—figure supplement 3B–F, and Figure 7—figure supplement 2.

^‡Measured reversal potential, in mV, under a 5-fold NaCl gradient (E_Cl = –40.2 mV). Extracted from data in Figure 1C and Figure 7—figure supplement 1A–H.

^§K_m and V_SCN/V_Cl values are derived from conductance–concentration data presented in Figure 1D, Figure 7, and Figure 7—figure supplement 3F–J.

^¶nd, not determined.