Fig. 2. Cortical actomyosin regulates cell decision-making in response to hydraulic resistance.

(A) Distribution pattern of MDA-MB-231 cells in branch channels of different hydraulic resistances in response to different pharmacological [(CK666 and Latrunculin A (LatA)] or molecular interventions (mDia1-KD) (n > 70 cells from three independent experiments for CK666 and mDia1-KD experiments and n > 20 cells from three independent experiments for LatA experiments). Data represent the mean with 95% confidence interval. P < 0.01 for the distribution of LatA-treated cells versus controls as indicated by χ² test. (B) Decision-making times of MDA-MB-231 cells in response to different pharmacological (CK666 and LatA) or molecular interventions (mDia1-KD) (n > 30 cells from three independent experiments for each condition). Data represent the means ± SD. Mann-Whitney U test was performed, ****P < 0.0001 relative to scramble control (SC). Kruskal-Wallis with post hoc Dunn was performed, ****P < 0.0001 relative to vehicle control (VC). (C) Distribution pattern of MDA-MB-231 cells in branch channels of different hydraulic resistances in response to different pharmacological (blebbistatin) or molecular interventions (myosin-IIA or myosin-IIB KD) (n > 70 cells from three independent experiments for each condition). Data represent the mean with 95% confidence interval. P < 0.01 and P < 0.0001 for the distribution of blebbistatin-treated and myosin-IIA–KD cells, respectively, relative to controls as indicated by χ² test. (D) Decision-making times of MDA-MB-231 cells in response to different pharmacological (blebbistatin) or molecular interventions (myosin-IIA or myosin-IIB KD) (n > 40 cells from three independent experiments for each condition). Data represent the means ± SD. Kruskal-Wallis with post hoc Dunn was performed, *P < 0.05 relative to scramble control. Mann-Whitney U test was performed, ****P < 0.0001 relative to vehicle control.