Table 1.
PCR primers used in this study
| Name | Primer sequence (5'→3')a | Purpose |
| rsbR-a | CCGGGATCCATACGGCCTGTTCTCATCATTC | Construction of rsbR null mutant |
| rsbR-b | ACGCGTTGTTTGTAGGTTTCTG | |
| rsbR-c | CAGAAACCTACAAACAACGCGTTACAGCCAGCAGTTGCGATTACAC | |
| rsbR-d | CCCAAGCTTTACAGATTTCTCCTCGTCCAG | |
| rsbR-up | GCGAGTGTACCCATGTCGAAGCAG | Screening of positive clones of rsbR null mutant |
| rsbR-dn | CCCCAATTCCTGTTTAAGTTTTTCCAG | |
| rsbR-W | ACGCGTCGACCAATCCAAGCAAAATAGCTAGGTAGAAA | Complementation of rsbR deletion |
| rsbR-X | CAATCCAAGCAAAATAGCTAGGTAGAAA | |
| rsbR-Y | CTAGCTATTTTGCTTGGATTGATGTATAAAGATTTTGCAAACTT | |
| rsbR-Z | CGAGCTCTCACCCCTCTTTTTCTACT | |
| rsbR175m-F | TGTAATGCCGTTAATTGGAACGATTGACGCAGAAAGAGCCAAG | Construction of mutant rsbR complementary plasmid |
| rsbR175m-R | AGTAAGTTTTCTATGATTAACTTGGCTCTTTCTGCGTCAATCGT | |
| rsbR209m-F | TGATTGATATTACGGGAGTTCCTGTTGTTGATGCAATGGTTGCG | |
| rsbR209m-R | GACGCTTGAATAATATGGTGCGCAACCATTGCATCAACAACAGG | |
| rsbR-RT-F | CGCGGATACGTGGGAAAAG | qRT-PCR |
| rsbR-RT-R | TGCCTGACAGCCGACAAGTCT |
a
Nucleotides introduced to create restriction sites and point mutations are underlined and in bold, respectively. qRT-PCR: quantitative reverse transcription PCR