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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Bioorg Chem. 2019 May 25;89:103014. doi: 10.1016/j.bioorg.2019.103014

Figure 2.

Figure 2.

Ability of coumarins and UVA light to damage plasmid DNA. Plasmid DNA was treated with increasing concentrations of the coumarins. Lane L shows products from the digestion of the plasmid with restriction endonuclease EcoRI to linearize the DNA (100 ng); in native gels, the linearized DNA migrates as one double-stranded band, whereas, in denaturing gels, it migrates as two well-separated single strands. Lane S shows untreated plasmid DNA (75 ng); in native gels, the intact plasmid DNA migrates mainly as one supercoiled double-stranded band, whereas in denaturing gels, it migrates as two closely migrating single strands. Plasmid was analyzed in neutral (panels A and B) or alkaline (panels C and D) agarose gels. Plasmid samples were treated with (panels B and D) or without (panels A and C) UVA light.