Table 2.

Percent linearization of plasmid DNA by coumarins plus UVA light

Concentration (μM)	Percentage of nicked plasmid
	Compd 14	Compd 48	Compd 58	Compd 62	Compd 71
0	0.2	0.2	1.0	1.2	2.2
1	1.0	4.0	3.2	3.2	6.8
10	8.0	60.5	12.0	55.4	80.2
100	20.5	90.8	20.2	96.6	98.6

Table 2. DNA nicking data from plasmids analyzed in neutral agarose gels (Figure 2, panel B). In these experiments, plasmids were treated with increasing concentrations of the coumarins followed by UVA light. Image analysis was used to calculate the percent of plasmid unwinding. Note that compounds 48, 62 and 71 were more effective in nicking DNA.