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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Jun 13;39(8):1602–1613. doi: 10.1161/ATVBAHA.119.312832

Figure 1. TG2 and FXIII activities, detected by SAs distribution, resemble their localization in ISs retrieved from E5.5 pregnant C57bl/6J mice.

Figure 1.

Myr-Venus homozygote males) were mated with C57bl/6J female mice, producing hemizygote Myr-Venus embryos. A. TG2 localization in paraffin sections of embryo IS. Red represents antibody staining for TG2 (4 dams, 20 ISs); B, C. TG2 activity, detected by T29-B distribution 45 min after it was injected IV (B, 4 dams, 14 ISs) or applied in situ on live sections (C, 4 dams, 16 ISs). For images B and C, red represents T29-B distribution, followed by Cy3-streptavidin staining; D. Negative control sections stained with Cy3-streptavidin staining without T29-B SA (5 dams, 20 ISs); E. FXIII distribution in paraffin sections of embryo IS (4 dams, 20 ISs). Red represents antibody staining for FXIII; F, G. FXIII activity, detected by F11-B distribution 45 min after it was injected IV (F, 5 dams, 17 ISs) or applied in situ on live sections (G, 4 dams, 11 ISs). For images F and G, red represents F11-B distribution, following Cy3-streptavidine staining; H. Negative control sections stained with Cy3-streptavidin staining without F11-B SA (5 dams, 20 ISs). For all images: green represents hemizygote Myr-Venus embryos; blue DAPI staining. Image scale bars are 200 μm.