. Author manuscript; available in PMC: 2019 Nov 20.

Published in final edited form as: Nat Microbiol. 2019 May 20;4(8):1401–1410. doi: 10.1038/s41564-019-0458-x

Table 1.

Cryo-EM data collection, refinement and validation statistics

	LAL14/1 pilus model (EMDB-0397) (PDB 6NAV)
Data collection and processing
Magnification	59,000
Voltage (kV)	300
Electron exposure (e–/Å²)	20
Defocus range (μm)	−1.5 to −2.5
Pixel size (Å)	1.4
Symmetry imposed	Helical (rise 4.94 Å, rotation 104.97°)
Initial particle images (no.)	881,252
Final particle images (no.)	181,113
Map resolution (Å)
model:map FSC (0.38)	4.1
”gold-standard” map:map FSC (0.143)	4.0
d₉₉	4.1

Refinement
Initial model used (PDB code)	N.A.
Map sharpening B factor (Å²)	−300
Model composition
Non-hydrogen atoms	891
Protein residues	131
Ligands	N.A.
B factors (Å²)
Protein	61.4
Ligand	N.A.
R.m.s. deviations
Bond lengths (Å)	0.005
Bond angles (°)	0.823
Validation
MolProbity score	1.52
Clashscore	4.1
Poor rotamers (%)	0
Ramachandran plot
Favored (%)	95.4
Allowed (%)	4.6
Disallowed (%)	0