Figure 2. Temporal dynamics of Copine RNA expression in postnatal retina.

Panels show in situ hybridization with probes against the 3’UTRs of four Copines predicted to be enriched in RGCs (Cpne4 = A-A’, Cpne5 = B-B’ Cpne6 = C-C’, Cpne9 = D-D’), the Brn3b gene (E-E’) and a negative control (F-F’). Experiments were performed on retinas from Brn3b^WT/WT (A-F) and Brn3b^KO/KO (A’-F’) mice at four different postnatal ages (P0, P3, P7, P14) and adult (between 2 and 6 months old). Retinal layers color code: NBL- blue, ONL- green, INL- yellow, GCL- pink. Note that Cpne4 is selectively expressed in the GCL beginning with P3, and specifically lost from the Brn3b^KO/KO retinas while Cpne5, Cpne6 and Cpne9 show varying degrees of enrichment in the GCL and INL, with Cpne9 being expressed at the highest levels, and essentially no regulation by Brn3b. Scale bar in F’ : 50μm. n=2–6 animals for each age for Brn3b^KO/KO as well as the age matched Brn3b^WT/WT.