Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 24;9:10759. doi: 10.1038/s41598-019-47257-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Cell quality of IPCs generated by the 3D cultured protocol was better than IPCs from conventional monolayer culture. (A) The cell viability of 3D cultured IPCs at day 21 was determined by FDP/PI staining. Green: FDP staining, red: PI staining, orange: merged image. Scale bar, 100 μm. (B) Absorbance at 450 nm showed significantly better cell viability of 3D cultured IPCs compared with IPCs from conventional 2D culture method. **P < 0.01, un-paired t-test. (C) Immunofluorescence of generated IPCs at day 21. Red: insulin, blue: DAPI. Scale bar, 100 μm. (D) The larger image shows the insulin-positive area was the cytoplasm. Red: insulin, blue: DAPI. (E) Light microscopy analysis also showed the cytoplasm of generated IPCs was positive for insulin staining. Scale bar, 10 μm. R: RCP petaloid μ-piece, stained blue. (F) MAFA mRNA expression was significantly higher in 3D cultured IPCs compared with IPCs from conventional 2D culture method by unpaired t-test. **P < 0.01. Error bar indicates standard deviation. (G) Generated IPCs did not show significant adverse effects on human dermal fibroblasts in cell toxicity, although 100 μM 5-FU addition showed significantly reduced viability. *P < 0.05, one-way ANOVA.