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. 2019 Jul 24;9:10759. doi: 10.1038/s41598-019-47257-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glucose stimulation index of IPCs generated by the 3D cultured protocol was significantly higher than IPCs generated by conventional 2D culture. The lines in the boxes show the median values, and the error bar shows the standard deviation. Unpaired t-test, **P < 0.01.