Fig. 2.
Combinatorial recognition of Cdc34Δdist by ScUba1. a Top middle, overall structure of Uba1-Cdc34Δdist/Ub as in Fig. 1a. Top left, network of contacts between Uba1 UFD and Cdc34 with involved residues shown as sticks with red oxygen atoms, blue nitrogen atoms, and yellow sulfur atoms. Hydrogen bonds are indicated by dashed lines. Top right, interaction network between Cdc34 and Uba1 SCCH. Bottom middle, tripartite network between Cdc34, Ub(a), and Uba1 crossover loop. b, c E1-E2 thioester transfer assays of the indicated mutants for Uba1 UFD (b) and SCCH (c). Data are represented by mean ± SD with representative replicates labeled above and individual replicates shown as gray circles. Biochemical assays have four independent replicates, unless indicated by an asterisk when there are three replicates. d Electrostatic surface of Uba1 UFD with Cdc34 contacting residues overlaid as in a. e E1-E2 thioester transfer assays of the indicated mutants for Cdc34 (E2), Uba1 UFD, and a charge reversal rescue assay with mutant Uba1 and Cdc34, represented as in b, c. Source data are provided as a Source Data file