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. 2019 Jul 24;9:10716. doi: 10.1038/s41598-019-47009-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Promoter-based cell-type targeting. (A) Diagram of cotransfection using the ubiquitous CAG promoter to drive HyPBase and integrating palmTagRFP reporter. (B) As in (A), using the mouse aMHC promoter to drive HyPBase. (C) Comparison of plasmid expression 16 hrs. and 160 hrs. post transfection. (D) Diagram of dissociation protocol. (E) Comparison of MF20+ (green), palmTagRFP+ cells between CAG-HyPBase and aMHC-HyPBase transfected cells, asterisks indicate non-myocytes (MF20-). Scalebar = 100 um (F) Quantification of the percentage of myocytes following dissociation and culture of CAG-HyPBase and aMHC-HyPBase transfected hearts (n = 5547 cells, 3 biological replicates per group). (G) Quantification of the percentage of palmTagRFP positive cells that were myocytes following dissociation and culture of CAG-HyPBase and aMHC-HyPBase transfected hearts (n = 387 cells, 3 biological replicates per group).