Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 24;9:10716. doi: 10.1038/s41598-019-47009-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Real-time analysis of cellular function. (A) PalmTagRFP localization in cells isolated from a heart cotransfected with a membrane reporter and the calcium reporter GCamp6F. ((B) GCamp6f localization (detected with an antiGFP antibody) in cells from (A). (C) Images from (A) and B) overlaid on a phase contrast image. (D) Higher magnification image of cell from (A-C) demonstrating GCamp6f protein distribution in transfected cell. (E) Time series of GCamp6f intensity in a pair of transfected cells isolated from an E4 heart. (F) Plot of calcium transient intensity at point of maximal activity in cells from (E). G) Calcium transient traces (dF/F0) measured from areas denoted by asterisks from (F). (H-I) As in (E-G), for cells isolated from an E9 heart. Note both the shape of the cells and the pattern of calcium transient activation has changed. (K) Image of a heart cotransfected with palmTagRFP and GCamp6F. (L) Time series of calcium transient intensity in the heart from (K) (see also Sup Movie 1). (M) Calcium transient traces (dF/F0) from an atrial (orange) and ventricular (blue) cell from (K).