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. 2019 Jul 16;28(3):723–734.e6. doi: 10.1016/j.celrep.2019.06.052

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© 2019 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Release of ABCE1 from the Small Ribosomal Subunit Is Followed by Site II Opening

(A) Cartoon summarizing the experimental settings.

(B and C) Release of ABCE1 from the small ribosomal subunit (black) occurring (B) only at the physiological temperature (70°C–80°C) and simultaneous ADP competition or (C) by 30S and AMP-PNP dilution at the indicated time points (manual mixing didn’t allow earlier time points), and its interdependence with site II opening (orange). Data represent mean ± SD from four independent experiments.