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Human epidermal growth factor receptor 2 (HER2) expression in the primary tumor (A) and metastatic lymph node (B) by flow cytometric analysis (gray dotted line: negative control, black solid line: trastuzumab). The tumor cells from both the primary tumor and metastatic lymph node were positive for HER2, and trastuzumab could bind to canine anal sac gland carcinoma cells.
