Table 5.

Data of metformin HCl Transferred through non-everted intestinal sac (Method one).

Perfusion solution A	r 2	Papp	Intercept	Total penetration %	Absorption enhancement %
Metformin	0.971	0.0010	0.0072	26.972 ± 1.21	100.000
Metformin + tween	0.971	0.0018	0.0127	47.159 ± 0.21	174.842
25% Span + tween	0.973	0.0034	0.0103	63.840 ± 0.61	236.687
33% Span + tween	0.984	0.0035	0.0105	66.520 ± 0.13	246.625
50% Span + tween	0.984	0.0035	0.0110	67.495 ± 0.22	250.238
66% Span + tween	0.977	0.0034	0.0117	67.143 ± 0.28	248.935
75% Span + tween	0.984	0.0035	0.0115	68.349 ± 0.35	253.404
Perfusion solution B
Metformin	0.988	0.0008	0.0026	16.212 ± 0.80	100.000
Metformin + tween	0.963	0.0012	0.0057	27.233 ± 0.36	167.978
25% Span + tween	0.995	0.0029	-0.0015	36.933 ± 1.36	227.808
33% Span + tween	0.996	0.0029	-0.0014	37.838 ± 1.10	233.387
50% Span + tween	0.997	0.0029	-0.0011	37.959 ± 1.35	234.136
66% Span + tween	0.994	0.0028	-0.0011	37.224 ± 0.56	229.599
75% Span + tween	0.994	0.0028	-0.0005	37.069 ± 0.41	228.643
Perfusion solution C
Metformin	0.971	0.0010	0.0072	26.972 ± 1.21	100.000
Metformin + tween	0.971	0.0018	0.0127	47.159 ± 0.21	174.842
25% Span + tween	0.984	0.0027	0.006	47.523 ± 0.27	176.193
33% Span + tween	0.980	0.0027	0.0061	47.637 ± 0.29	176.616
50% Span + tween	0.982	0.0027	0.0064	48.058 ± 0.58	178.175
66% Span + tween	0.987	0.0028	0.0061	48.009 ± 0.69	177.993
Perfusion solution D
Metformin	0.971	0.0010	0.0072	26.972 ± 1.21	100.000
Metformin + tween	0.971	0.0018	0.0127	47.159 ± 0.21	174.842
25% Span + tween	0.970	0.0033	0.0026	45.623 ± 0.69	169.149
33% Span + tween	0.961	0.0033	0.0029	46.673 ± 1.89	173.040
50% Span + tween	0.958	0.0033	0.0024	45.076 ± 0.53	167.119
66% Span + tween	0.977	0.0032	0.0033	47.496 ± 1.77	176.092