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. 2019 Jul 24;9:10707. doi: 10.1038/s41598-019-47077-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Plots of mRNA. Scatter plot (A), the x-axis and y-axis represent the mean FPKM values (log2 transformation) of each group of sample genes. The two oblique lines divide the upper and lower genes (1.5 fold difference) and the unmodified genes. Volcano-Plot (B), the x-axis represents the log2Fold_Change value and the y-axis represents -log10p_value. The vertical two green lines are up (right) and down (left), and the green parallel lines correspond to p-value. Hierarchical clustering (C) was performed using FPKM values of significant expression genes obtained by comparison between groups, each row representing one gene and each column representing one sample. The red dots represent up-regulated DEgenes, the green dots represent down-regulated DEgenes, and the gray dots represent indistinguishable genes. PCA analysis (D), each color represents each grouping, and each axis represents a principal component.