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. Author manuscript; available in PMC: 2019 Jul 25.
Published in final edited form as: Science. 2019 Mar 29;363(6434):eaat9691. doi: 10.1126/science.aat9691

Fig. 3. Pf phage inhibits TNF in a type I IFN-dependent manner.

Fig. 3.

(A) TNF production by murine BMDCs stimulated with alginate and Pf4 for 48 hours. (B) TNF production over time by murine BMDCs stimulated with alginate and Pf4. (C) TNF production in BMDCs stimulated with alginate and either Pf4 or Pf1 phage for 72 hours. (D) TNF production in BMDCs stimulated with LPS and Fd1 phage for 24 hours. (E) TNF mRNA up-regulation in BMDCs stimulated with LPS and Pf4 for various time points. (F) Intracellular cytokine staining of TNF in BMDCs stimulated with Pf4 and then LPS. (G) Type I IFN production by BMDCs stimulated with alginate and Pf4 for 24 hours. (H) TNF production by WT or Ifnar−/− BMDCs stimulated with LPS and Pf4 phage for 24 hours. (A) to (H) are each representative of n ≥ 3 experiments and depict mean with SEM of n ≥ 3 replicates. Statistics: (E, G to H) two-tailed Student’s t test; (A, C to D) one-way ANOVA with Dunnett’s multiple comparison; (B) two-way ANOVA.