Figure 5.

Experimental Tumors Generated from PDAC Cell Lines Expressing Candidate miRNAs from the miR-200 Family

Panc-1 cells were transduced with lentiviral vectors encoding for GFP (A, D, G, and J), miR-200a-b-429 (B and H), miR-200c-141 (C and I), miR-141 (E and K), or miR-429 (F and L) and implanted in the pancreas of athymic mice as described in the Materials and Methods. Mice were sacrificed, and tumors were sampled and stained using H&E. (A–F) Primary tumors. (G–L) Metastatic growth. Results are representative of 13 mice per group.