Figure 6.

In Vivo Activity of miR-200 Family Members on Experimental Tumor Growth and Dissemination

PANC-1 cells transduced with miR-200b-a-429, miR-200c-141, miR-141, miR-429, or control GFP cells were injected orthotopically in nude mice (n = 13 per group), as described in the Materials and Methods. (A and B) Illustrative pictures of the orthotopic grafts (A) and liver metastases (B) obtained from animals of the control group. (C) Number of mice with orthotopic grafts (left) and distal metastases (right) are indicated among the different study groups. (D) Bar graph of the orthotopic tumor volume (in mm³). (E–I) Expression levels of mIR-200 family (E, miR-141; F, miR-429) and target genes (G, ZEB1; H, ZEB2; and I, CDH1) (median of 2^−ΔCT) in tumors generated from cell lines transduced with miR-141 or miR-429, or with GFP-encoding vector as a control.