Fig. 3.

Effect of The Propolis Samples on The Growth Factors-Enhanced HUVECs. HUVECs were treated with VEGF (20 ng/ml) and bFGF (50 ng/ml), and also the various concentration of (a) D01 and (b) D02. After 48 hours of incubation, 10μl/well of CCK-8 solution was added and the absorbances at 450nM were measured. These data are compared with the absorbance of HUVECs treated without growth factors but with the same concentration of propolis samples, which have similar graphic trends with HUVEC data in Fig. 2. Data are expressed as absorbance values with normalization to the basal values of cells with DMSO as the control and represent biological replicates. The experiment was repeated two times with five technical replicates in each experiment.