Table 1.
Details on target gene, primer sequences, amplicon lengths of the RoTat 1.2 PCR and the EVAB PCR.
Taxon | Target gene | Primers | Primer sequences | Amplicon length | Reference |
---|---|---|---|---|---|
T. evansi type A | RoTat 1.2 VSG | ILO7957 | 5′- GCC ACC ACG GCG AAA GAC -3′ | 488 bp | Urakawa et al. (2001) |
ILO8091 | 5′-TAA TCA GTG TGG TGT GC-3′ | ||||
T. evansi type B | minicircle | EVAB-1 | 5′-ACAGTCCGAGAGATAGAG-3′ | 436 bp | Birhanu et al. (2015) |
EVAB-2 | 5′-CTGTACTCTACATCTACCTC-3′ |