Table 2.
Differentially expressed genes related to energy metabolism, muscle structure, and stress response
| Genea | Description | Fold changeb | ||
|---|---|---|---|---|
| 35AL vs. 22AL | 22PF vs. 22AL | 35AL vs. 22PF | ||
| Lipid metabolic process | ||||
| ACSL1 | Acyl-CoA synthetase long-chain family member 1 | 0.22 | 0.45 | |
| ITGAV | Integrin, alpha V | 0.25 | ||
| CD36 | CD36 molecule | 0.28 | ||
| FABP3 | Fatty acid binding protein 3 | 0.42 | 0.46 | |
| DLD | Sus scrofa dihydrolipoamide dehydrogenase | 0.22 | 0.29 | |
| CPT1B | Carnitine palmitoyltransferase 1B | 0.48 | ||
| LPL | Lipoprotein lipase | 0.35 | ||
| SCD | Stearoyl-CoA desaturase | 0.18 | ||
| DECR1 | 2,4-Dienoyl CoA reductase 1, mitochondria | 0.24 | 0.50 | 0.50 |
| ACADL | Acyl-CoA dehydrogenase, long chain | 0.47 | 0.39 | |
| ACADM | Acyl-CoA dehydrogenase | 0.39 | 0.46 | |
| ACOX1 | Acyl-CoA oxidase 1, palmitoyl | 0.44 | ||
| CYP2E1 | Cytochrome P450, family 2, subfamily E, polypeptide 1 | 6.22 | 3.79 | |
| ATP5B | ATP synthase, H+ transporting, mitochondrial F1 complex | 0.21 | 0.41 | |
| FASN | Sus scrofa fatty acid synthase | 2.42 | ||
| APOC3 | Sus scrofa apolipoprotein C-III | 4.99 | 0.29 | |
| CPT1A | Sus scrofa carnitine palmitoyltransferase 1A | 0.45 | ||
| LPINI | Sus scrofa lipin 1 mRNA | 0.35 | 0.38 | 0.72 |
| PPARGC-1 | Sus scrofa peroxisome proliferator activated receptor gamma, coactivator 1 alpha (PPARGC-1) | 2.37 | ||
| Glucose metabolic process | ||||
| UGP2 | UDP-glucose pyrophosphorylase 2 | 0.16 | 0.40 | |
| GPI | Glucose-6-phosphate isomerase | 0.20 | 0.34 | |
| GPD1 | Glycerol-3-phosphate dehydrogenase 1 | 0.38 | 0.44 | |
| PFKM | Phosphofructokinase, muscle | 0.36 | 0.45 | |
| TPI1 | Triosephosphate isomerase 1 | 0.15 | 0.23 | |
| PKM2 | Pyruvate kinase, muscle | 0.14 | 0.23 | |
| PGM1 | Phosphoglucomutase | 0.35 | 0.33 | |
| PCK1 | Sus scrofa phosphoenolpyruvate carboxykinase 1 | 2.42 | 2.42 | |
| Muscle structure development | ||||
| SHOC2 | Soc-2 suppressor of clear homolog | 0.32 | 0.45 | |
| CASQ1 | Calsequestrin 1 (fast-twitch, skeletal muscle) | 0.46 | 0.46 | |
| MYH4 | Myosin, heavy chain 4, skeletal muscle | 0.29 | 0.43 | |
| ACTA1 | Actin, alpha 1, skeletal muscle | 0.32 | 0.36 | |
| ACTA2 | Actin, alpha 2, smooth muscle, | 0.39 | 0.40 | |
| ACTC1 | Actin, alpha, cardiac muscle 1 | 0.435 | 0.26 | |
| BIN1 | Bridging integrator 1 | 0.17 | 0.16 | |
| HMGB1 | High mobility group box 1 | 0.45 | 0.34 | |
| SGCA | Sarcoglycan, alpha | 0.25 | 0.40 | |
| ITGB1 | Integrin, beta 1 | 0.18 | 0.27 | |
| CTNNB1 | Catenin (cadherin-associated protein), beta 1 | 0.38 | 0.49 | |
| MSTN | Myostatin | 0.41 | 1.14 | 0.36 |
| MYO1B | Myosin IB | 0.349 | 0.42 | |
| PPP3CB | Protein phosphatase 3, catalytic subunit, beta isozyme | 0.18 | 0.28 | |
| CAV2 | Caveolin 2 | 0.25 | 0.45 | |
| TPM2 | Tropomyosin 2 (beta) | 0.33 | 0.36 | |
| TNNT3 | Troponin T type 3 (skeletal, fast) | 0.39 | 0.45 | |
| TNNI1 | Troponin I type 1 (skeletal, slow) | 0.37 | ||
| LMNA | Prelamin-A/C | 0.27 | 0.44 | |
| MYOT | Sus scrofa myotilin | 0.40 | 0.44 | |
| TPM3 | Sus scrofa tropomyosin 3 | 0.41 | ||
| TNNI3 | Troponin I type 3 | 0.39 | 0.42 | 0.45 |
| Cellular response to stress | ||||
| GTF2H4 | General transcription factor IIH subunit 4 | 2.13 | ||
| ERCC1 | Excision repair cross-complementing rodent repair complementation group 1 | 2.06 | ||
| HSPA1L | Heat shock 70 kDa protein 1-like | 2.20 | 2.64 | |
| MLH1 | mutL homolog 1 | 2.09 | ||
| GADD45A | Growth arrest and DNA-damage-inducible, alpha | 2.04 | ||
| CDKN1A | Cyclin-dependent kinase inhibitor 1A | 3.78 | ||
| MB | Sus scrofa myoglobin (MB) | 0.12 | 0.25 | |
| CXCL10 | Chemokine (C-X-C motif) ligand 10 | 2.58 | ||
| MAPK14 | Mitogen-activated protein kinase 14 | 3.02 | 2.11 | |
| TICAM2 | Toll-like receptor adaptor molecule 2 | 3.35 | 2.02 | |
| Cellular response to hormone stimulus | ||||
| NR3C1 | Nuclear receptor subfamily 3, group C, member 1 | 0.48 | ||
| GHR | Growth hormone receptor | 0.27 | ||
| ACVR1 | Activin type I receptor | 0.30 | ||
| ADRB2 | Adrenergic, beta-2- receptor | 0.32 | 0.49 | |
| IFNGR2 | Interferon gamma receptor 2 | 0.27 | ||
| FOLR2 | Folate receptor 2 | 0.47 | 0.44 | |
| ADIPOR1 | Adiponectin receptor 1 | 0.48 | ||
aGenes shown in boldface were used in qPCR verification
bOnly the fold change of differentially expressed genes related to energy metabolism, muscle structure, and stress response are shown. Values > 1.0 show upregulation, and < 1.0 show downregulation