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. 2019 Jul 24;19:192. doi: 10.1186/s12935-019-0914-y

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — CC-CAFs enhanced adhesion and metastasis of CRC cells in vivo. a Liver tissue from BALB/c (nu/nu) mice of each group was obtained at 6 weeks, the number of liver metastatic foci was counted. And weight of liver was quantified. *P < 0.05, **P < 0.01. b Lung tissue from BALB/c (nu/nu) mice of each group was obtained at 8 weeks, the number of lung metastatic foci was counted. And weight of lung was quantified. *P < 0.05, **P < 0.01. c 1 × 10⁶ SW48-GFP cells were injected via tail vein, and 48 h later lungs were taken out for frozen sections. The count of sw48-GFP with or without transfection of shRNA CD44 or pre-co-cultured with CC-CAFs in frozen section per field was analyzed (magnification, ×100). Error bars represent mean ± s.d; **P < 0.01; ***P < 0.001; n.s not significant; by one-way analysis of variance (ANOVA)