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. 2019 Jun 24;10(6):222–233. doi: 10.5306/wjco.v10.i6.222

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©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Dose-response effects of leptin on the levels of Notch mRNA in endometrial cancer cells. A, B: Notch mRNA levels (receptors: Notch1, Notch2, Notch3 and Notch4; ligands: JAG1 and DLL4 and targets: Survivin, Hey2) induced by leptin (0, 0.6, 1.2 and 6.2nM) in type I endometrial cancer (EmCa) (A, Ishikawa) and type II EmCa cells (B, An3Ca). Cells were cultured for 24h. Notch mRNA levels were quantified by real-time RT-PCR. Cells were cultured for 24h. RNA expression was calculated by normalizing values to GAPDH mRNA. Data (mean ± SE) representative results derived from a minimum of 3 independent experiments. ^cP < 0.05 and ^dP < 0.01 vs basal (no leptin).