Table 2.
The Primer Sequences for qRT-PCR Analysis
| Gene | Direction | Sequence |
|---|---|---|
| Circ-UBE2D2 | Forward | AATGGCAGCATTTGTCTTGA |
| Reverse | GCCCCTGTGAGTAAGCTACG | |
| miR-1236 | Forward | CAGTGAGTGACAGGGGAA |
| Reverse | CCAGTTTTTTTTTTTTTTTCCCCAT | |
| miR-1287 | Forward | GTGCTGGATCAGTGGTTC |
| Reverse | GTCCAGTTTTTTTTTTTTTTTGACTC | |
| HOXB7 | Forward | CGTCCCTGCCTACAAATCAT |
| Reverse | GCAAACGCACAAGAAGTTTG | |
| MTA2 | Forward | TTTCACGCCATGGATACCTT |
| Reverse | AGGGCCTCCTCAAATAGCAT | |
| ZEB1 | Forward | CACCAGATGCATTTTCACAA |
| Reverse | GTGTAACTGCACAGGGAGCA | |
| KLF8 | Forward | GGTGTCCACGTCAACATCTG |
| Reverse | CATGGGCAGAGACTGCACTA | |
| AFP | Forward | GCCTTTCTGGAAGAACTTTGC |
| Reverse | CTGGAGTGGGCTTTTTGTGT | |
| p21 | Forward | GACACCACTGGAGGGTGACT |
| Reverse | CAGGTCCACATGGTCTTCCT | |
| PIK3CB | Forward | ACCCAATGTTCAACCTCCTT |
| Reverse | CCCTGGGTCACAACTTCTTG | |
| ANGPT1 | Forward | GAGAACCTTCAAGGCTTGGTT |
| Reverse | GACTGTGTCCATCAGCTCCA | |
| GAGE1 | Forward | GAACCAGCAACTCAACGTCA |
| Reverse | TTCACCTCCTCTGGATTTGG | |
| CD105 | Forward | CATCCGTCTCCGAGTTCCT |
| Reverse | ACAGCAGGCTCACACAGTTG | |
| ATF6α | Forward | GGGAGTCACACAGCTCCCTA |
| Reverse | TGAACCGACATTTCTCATGG | |
| EGFR | Forward | TTACGCTCCCTCAAGGAGAT |
| Reverse | TGCAGCTGTTTTCACCTCTG | |
| GAPDH | Forward | ACCCAGAAGACTGTGGATGG |
| Reverse | TTCAGCTCAGGGATGACCTT | |
| U6 | Forward | CTCGCTTCGGCAGCACA |
| Reverse | AACGCTTCACGAATTTGCGT |