Figure 1.

Treatment with TGF-β leads to a switch from epithelial to mesenchymal cell marker in the osteosarcoma cell line, which can be inhibited by pharmacological inhibition of PKC. A – Immunoblot analysis of indicated markers in cell lysates obtained from DAN cells treated with TGF-β for 72 h. The blots were stripped and re-probed with anti-β-actin to confirm equal loading. Representative blots from three independent experiments are shown. B – Immunofluorescence analysis of E-cadherin and P-Smad2/3 in DAN cells treated with TGF-β for 72 h and with 10 nM of Go6983 or DMSO in hours 48–72. Scale bar: 10 μm. Images from three independent experiments are shown. C – Immunoblot analysis of indicated markers in cell lysates obtained from DAN cells treated with TGF-β for 72 h and with 10 nM of Go6983 or DMSO from hours 48–72. The blots were stripped and re-probed with anti-β-actin to confirm equal loading