Extended Data Table 1.

Summary of RNA edits observed for all RNA-seq experiments.

Figures	Cell	BE	gRNA	Sort	Replicate	C-to-U (for CBE) orA-to-l(forABE)	Other	C-to-U orA-to-l (%)
Fig. 1c	HepG2	GFP	--	MFI-matched to top 5% BE3 expression	Rep. 1	8	46	14.815
Fig. 1c	HepG2	BE3	RNF2	Top 5%	Rep. 1	58,372	8	99.986
Fig. 1c & Ext. Data Fig. 7b					Rep. 2	77,671	7	99.991
					Rep. 3	60,225	7	99.988
					Rep. 4	61,736	3	99.995
Fig. 2a	HEK293T	BE3	RNF2	Top 5%	Rep. 1	91,951	9	99.990
					Rep. 2	192,088	6	99.997
					Rep. 3	90,509	3	99.997
	HEK293T	BE3-R33A	RNF2	Top 5%	Rep. 1	236	94	71.515
					Rep. 2	377	126	74.950
					Rep. 3	197	63	75.769
	HEK293T	BE3-R33A/K34A	RNF2	Top 5%	Rep. 1	24	89	21.239
					Rep. 2	26	128	16.883
					Rep. 3	7	47	12.963
	HEK293T	BE3-E63Q	RNF2	Top 5%	Rep. 1	15	51	22.727
					Rep. 2	9	47	16.071
Fig. 3b	HEK293T	GFP	--	MFI-matched to top 5% BE3 expression	Rep. 1	195	138	58.559
Fig. 3b	HEK293T	ABEmax	HEK site 2	Top 5%	Rep. 1	37,061	88	99.763
					Rep. 2	31,821	67	99.790
					Rep. 3	28,752	49	99.830
Ext. Data Fig. 2c	HEK293T	BE3	RNF2	All GFP	Rep. 1	28,197	12	99.957
					Rep. 2	29,577	28	99.905
					Rep. 3	44,435	19	99.957
	HEK293T	BE3	EMX1	All GFP	Rep. 1	31,270	11	99.965
					Rep. 2	27,811	41	99.853
					Rep. 3	34,679	14	99.960
Ext. Data Fig. 4c	HEK293T	BE3	RNF2	Top 5%	Rep. 1	159,416	8	99.995
					Rep. 2	140,530	11	99.992
	HEK293T	BE3	EMX1	Top 5%	Rep. 1	137,925	4	99.997
					Rep. 2	110,930	5	99.995
	HEK293T	BE3	NT	Top 5%	Rep. 1	142,143	25	99.982
					Rep. 2	147,912	8	99.995
Ext. Data Fig. 6a	HEK293T	BE3	RNF2	Top 5%	Screen	69,623	31	99.955
		BE3-E63Q				76	270	21.965
		BE3-P29F				63	228	21.649
		BE3-P29T				300	231	56.497
		BE3-L182A				2,128	332	86.504
		BE3-R33A				435	283	60.585
		BE3-K34A				5,665	196	96.656
		BE3-R33A/K34A				63	173	26.695
Ext. Data Fig. 7b (also includes WT BE3 from Fig. 1c)	HepG2	BE3-R33A	RNF2	Top 5%	Rep. 1	41	54	43.158
					Rep. 2	44	30	59.459
					Rep. 3	76	32	70.370
	HepG2	BE3-R33A/K34A	RNF2	Top 5%	Rep. 1	6	35	14.634
					Rep. 2	5	32	13.514
					Rep. 3	7	27	20.588