TABLE 1.

Universal and fungal specific primers for the dual DNAbarcoding scheme.

Barcode	Primer name	Sequence	References
Primary barcode	ITS1	5′ TCCGTAGGTGAACCTGCGG 3′	White et al.,1990
	ITS2	5′ GCTGCGTTCTTCATCGATGC 3′	White et al.,1990
	ITS3	5′ GCATCGATGAAGAACGCAGC 3′	White et al.,1990
	ITS4	5′ TCCTCCGCTTATTGATATGC 3′	White et al.,1990
	ITS5	5′ GGAAGTAAAAGTCGTAACAAGG 3′	White et al.,1990
	ITS1F	5′ CTTGGTCATTTAGAGGAAGTAA 3′	Gardes and Bruns,1993
	IT2	5′ CCTCCGCTTATTGATATGCTTAGG 3′	Beguin et al.,2012
	SR6R	5′ AAGTATAAGTCGTAACAAGG 3′	Vilgalys and Hester,1990
	LR1	5′ GGTTGGTTTCTTTCCT 3′	Vilgalys and Hester,1990
	V9D	5′ TTAAGTCCCTGCCCTTTGTA 3′	Gerrits van den Ende and de Hoog,1999
	V9G	5′ TACGTCCCTGCCCTTTGTA 3′	Gerrits van den Ende and de Hoog,1999
	LS266	5′ GCATTCCCAAACAACTCGACTC 3′	Gerrits van den Ende and de Hoog,1999
	NL4b	5′ GGATTCTCACCCTCTATGAC 3′	O’Donnell,1993
Secondary barcode	EF1-1002F	5′ TTCATCAAGAACATGAT 3′	Stielow et al.,2015
	EF1-1018F	5′ GAYTTCATCAAGAACATGAT 3′	Stielow et al.,2015
	EF1-1620R	5′ GACGTTGAADCCRACRTTGTC 3′	Stielow et al.,2015
	EF1-1688R	5′ GCTATCATCACAATGGACGTTCTTGGAG 3′	Stielow et al.,2015
	EF1-983F	5′ GCYCCYGGHCAYCGTGAYTTYAT 3′	Rehner and Buckley,2005
	EF1-1567R	5′ ACHGTRCCRATACCACCRATCTT 3′	Rehner and Buckley,2005