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. Author manuscript; available in PMC: 2019 Jul 25.
Published in final edited form as: J Phys Chem Lett. 2019 Jun 13;10(13):3563–3570. doi: 10.1021/acs.jpclett.9b01289

Table 1.

Spectral properties of commercial red SREF dyes experimented in this study.

Dye1 Solvent2 λabs (nm) C=C mode (cm−1) (ωp - ωs) + ωp
(nm)		 Concentration
(nM)		 SNR3
ATTO 610* a 621 1644 638.1 500 undetectable
Alexa 633^ a 630 1651 637.6 100 2.5
ATTO 610* b 631 1644 638.1 500 3.0
ATTO Rho14* a 632 1652 &1680 637.5 & 635.7 < 2000 7.5 & 2.1
Nile Blue A c 635 1652 637.5 500 7.1
ATTO 633* a 635 1640 638.4 500 3.1
ATTO Rho14* b 638 1652 &1680 637.5 & 635.7 50 3.0 & 1.5
Alexa 633^ b 640 1651 637.6 10 3.0
ATTO 633* b 642 1640 638.4 500 1.6
Alexa 647* a 654 1602 640.8 500 undetectable
JF 646* d 662 1616 639.9 500 undetectable
1.

^ for carboxy linker; * for NHS linker.

2.

a for PBS (pH =7.4); b for DMSO; c for ethanol; d for 0.1% TFA in ethanol.

3.

SNR for signal to noise ratio. data shown in Fig. 2, 3, 4.