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. Author manuscript; available in PMC: 2019 Jul 25.
Published in final edited form as: Cell Rep. 2019 May 21;27(8):2354–2369.e7. doi: 10.1016/j.celrep.2019.04.077

Figure 3. Hand2 Reorganizes Chromatin Accessibility toward a Pacemaker-like State.

Figure 3.

(A) ATAC-seq was performed on control, GMT-infected, and GHMT-infected MEFs, and P0 Hcn4-GFP+ endogenous pacemaker (P0 PM) cells were profiled for comparison. Unsupervised hierarchical clustering separated chromatin peaks into five clusters (1–5).

(B) Gene ontology (GO) term analysis of clusters 3 and 5.

(C) Known transcription factor motifs identified for clusters 3 and 5.

(D) RNA-seq enrichment of candidate transcription factors (TFs) for clusters 3 and 5.

(E) Cartoon diagram of the developmental pacemaker gene regulatory network (right) and associated RNA-seq data (left).

(F) Immunocytochemistry (ICC) on GHMT-reprogrammed cells for Tbx3 (top) and Shox2 (bottom). Scale bar, 20 mm.