Figure 2.

Co-culture of γδ T cells with autologous neutrophils in the presence of PDAC enhances cytotoxicity. (A) Cytotoxicity induced by short-term activated γδ T cells (γδ) at the indicated Effector/Target ratio (E/T). The mean of three individual samples for the indicated time points are shown. (B) Cytotoxicity induced by neutrophils (N) at the indicated Effector/Target ratio (E/T). E/T ratio was 12.5:1 (dashed line with x, 62.500 effector cells), 25:1 (dashed line with triangle, 125,000 effector cells) and 50:1 (solid line with square, 250,000 effector cells). The mean of eight individual samples for the indicated time points are presented. (C) Cytotoxicity of 250 x 10³ neutrophils (N, green line) or 125 × 10³ γδ T cells (γδ, blue line, E/T 25:1) alone or in combination (γδ + N, red line) against Panc89 cells was measured by RTCA. The mean of 15 individual samples for the indicated time points are shown. Percentage of lysis was analyzed from RTCA data by calculating the normalized impedance of spontaneous lysis (cell growth of tumor cells in medium alone) in relation to the maximal lysis induced by 1% Triton-X-100 (T-X-100, black line) at indicated time points. (A–C) Statistical analysis was performed by t-test. Significances are presented as P-Value; ^**P < 0.01 and ^*P < 0.05.