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. 2019 Jul 18;10:674. doi: 10.3389/fgene.2019.00674

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Copyright © 2019 Li, Li, Li, Ni, Xu, Yao, Wei, Zhang, Li, Zhao, Liu, Ullah, Jiang and Hu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of VRTN gene expression levels in different fetal stages for two spine traits. (A) Legend of spine traits during fetal development: T14 (top) and T13 (bottom). (B) Reverse transcription Polymerase Chain Reaction (RT-PCR) amplification of β-actin and VRTN (on the left is T13 and on the right is T14, both from the same period of fetal sheep). The order of electrophoresis is marker (TaKaRa DL500; 500, 400, 300, 200, 150, 100, and 50 bp), negative control, β-actin, and VRTN. (C) Heat maps of VRTN gene expression in T13 (top) and T14 (bottom) fetuses (p < 0.05). Red indicates high expression, and green indicates low expression. β-Actin was used as a control. The numbers on either side represent the length of the fetus. The unit is mm.