. 2019 May 16;24(4):749–761. doi: 10.1007/s12192-019-01001-2

Table 2.

List of antibodies used for Western immunoblotting (WB) and immunofluorescence (IF)

Antibody	Company	Host species	WB dilution	IF dilution
Pax7	Developmental Studies Hybridoma Bank, University of Iowa, IA, USA	Mouse monoclonal IgG1	1:100
Myogenin	Santa Cruz Biotechnology, Dallas, TX, USA	Mouse monoclonal IgG1	1:400
Sarcomeric MyHC (MF20)	Developmental Studies Hybridoma Bank, University of Iowa, IA, USA	Mouse monoclonal IgG2b	1:5000
Hsp25	Enzo Life Sciences, Farmingdale, NY, USA	Rabbit polyclonal IgG	1:5000	1:100
Hsp40		Rabbit polyclonal IgG
Hsp60		Goat polyclonal IgG
Hsp70/72		Rabbit polyclonal IgG
Hsp90		Rabbit polyclonal IgG
Anti-mouse HRP	GE Healthcare Life Sciences, Marlborough, MA, USA	Sheep	1:5000
Anti-rabbit HRP	GE Healthcare Life Sciences, Marlborough, MA, USA	Donkey	1:5000
Anti-goat HRP	Abcam, Cambridge, MA, USA	Donkey	1:5000
Alexa Fluor 488 anti-rabbit IgG	Thermo Fisher Scientific, Waltham, MA, USA	Goat		1:1000
Alexa Fluor 488 anti-goat IgG	Thermo Fisher Scientific, Waltham, MA, USA	Donkey		1:1000