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. 2019 May 27;24(4):719–733. doi: 10.1007/s12192-019-00996-y

Table 1.

Primer list of selected DEGs and DEMs in RT-qPCR verification experiments

Genes/miRNA Forward/reverse primer (5′-3′)
Calcium-transporting ATPase (Ca-ATP) F: 5′CGGTACTGGTGACAATAGAA3′
R: 5′GAGGATGACAAAGTGGAGC3′
V-TYpe H+-transporting ATPase subunit alpha (H-ATP) F: 5′AGAATATCCGAGTCCACG3′
R: 5′ACAAGACCACATCCCAAC3′
Nicotinic acetylcholine receptor subunit alpha-9 (CHRNA9) F: 5′ACTCCCTCTACAGATGCG3′
R: 5′TGGTGCCACTAAGGTGAA3′
Sodium- and chloride-dependent GABA transporter 2 (SLC6a8) F: 5′GACCAAAGTTACTGCTCCAC3′
R: 5′TTACCGTTTACCCGTGCC3′
Sodium-/potassium-transporting ATPase subunit alpha (NKA) F: 5′GTCCAACAGGGCATGAGT3′
R: 5′TGAGTGGGTACATACGAAGT3′
Calcium-activated chloride channel regulator 4-like (CLCA4) F: 5′CGTATGTTCGTATTTCTCCCTC3′
R: 5′ATGGCTACCATCCGGTCT3′
Glycine receptor subunit alpha-4 F: 5′AACCCGTGGTAGTGGTGG3′
(Glra4a) R: 5′TTCCCTGCTGGTCCTCAT3′
Potassium channel tetramerization domain 6 F: 5′TGTCGGCGGTAACTTCTA3′
(KCTD6) R: 5′TTGTGGGTACGATGAGCT3′
NADH dehydrogenase (Ubiquinone) 1 ą Subcomplex, 1 (NDUFA1) F: 5′TGCCTCTGAACGGGGAAAC3′
R: 5′CCAGCCAGCATAGTACCTGTAA3′

Aquaporin-9

(AQP9)

5′AGGCACCGACTACAGAAC3′
5′CACCTCTTAATCCAGCAC3′
β-actin F: 5′CGGCTGTGGTGGTGAAGGAGTA3′
R: 5′TCATGGACTCAGGAGACGGTGTG3′
miR-2010 GGGGTTACTGTTGATGTCAGCCCCTT
miR-2011 GGGACCAAGGTGTGCTAGTGATGAC
miR-2013 CGTGCAGCATGATGTAGTGGTGT
miR-10 GCGAACCCTGTAGATCCGAATTTGTG
miR-92b-3p TATTGCACTTGTCCCGGCCT

miR-278-3p

U6

GTCGGTGGGACTTTCGTTCGATT
ACGCAAATTCGTGAAGCGTT