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. 2019 Jul 18;13:295. doi: 10.3389/fncel.2019.00295

FIGURE 7.

FIGURE 7

Short-term plasticity is rescued by pretreatment with ANDRO. Representative traces (A) of two evoked fEPSPs separated by 50 ms before (pre-TBS) and after LTP induction (post-TBS) are shown. The graph (B) shows the ratio of the second fEPSP slope to the first fEPSP slope (R2/R1), recorded at different interval times: 10, 20, 30, 50, 60, 80, 100, 150, and 200 ms. WT (black dots), J20 (gray squares) and J20+ANDRO (red triangles). The values are expressed as mean ± SEM [two-way repeated measures ANOVA: interaction: F(12,105) = 1.09, p > 0.05; animal group factor: F(2,105) = 19.87, p < 0.001; ISI: F(6,105) = 18.58, p < 0.001, followed by Bonferroni post hoc test: WT vs. J20 at 10, 20 and 30 ms ∗p < 0.05, beyond p = n.s.; J20 vs. J20+Andro at 10 ms ∗∗∗p < 0.001, at 20 ms ∗∗p < 0.01, beyond p = n.s.; WT vs. J20+Andro p = n.s.]. For the same stimulus point, the three curves were compared (C) show the relative facilitation during the baseline period [(R2 – R1)/R1], pre-TBS, left plot (WT: 2.3 ± 0.5; J20: 0.7 ± 0.2; J20+ANDRO: 1.9 ± 0.4; one-way ANOVA, p < 0.05, followed by Bonferroni post hoc test, ∗p < 0.05, ∗∗p < 0.01) and after LTP induction [(R2’ – R1’)/R1’], post-TBS, right plot (WT: 1.9 ± 0.4; J20+ANDRO: 1.4 ± 0.3; J20: 0.6 ± 0.2, one-way ANOVA, p < 0.05, followed by Bonferroni post hoc test, ∗p < 0.05). The average of each bar was calculated over all the points during the baseline period and the last 20 min recorded during LTP. Also shown (D) are the same data as in (C), but plotted by animal group. Comparing facilitation within each group per separated, we showed that facilitation pre-TBS is significantly different than facilitation post-TBS in WT [two-way repeated measures ANOVA: interaction: F(2,14) = 2.68, p > 0.05; pre and post TBS condition: F(1,14) = 18.33, p < 0.001; animal group factor: F(2,14) = 8.43, p < 0.01, followed by Bonferroni post hoc test, WT pre vs. post TBS ∗p < 0.05, J20 pre vs. post TBS p > 0.05, J20+Andro pre vs. post ∗∗p < 0.01]. Plot of R1’ and R2’ (%) and the percentage of attenuation [(R2’ – R1’)/R1’] (E), revealing a reduced degree of response attenuation in J20 during the first 30 min after LTP induction (one-way ANOVA, p < 0.001 followed by Bonferroni post hoc test, WT vs. J20 ∗∗∗p < 0.001, J20 vs. J20+Andro ∗∗∗p < 0.001, WT vs. J20+Andro ∗∗p < 0.01). Data represents the mean ± SEM from 16 slices of 8 (WT) mice; 18 slices of 6 (J20) mice; 18 slices of 6 (J20+Andro) mice.