Fig. 6.

CK1 inhibition-induced destabilization of LRRK2 protein also occurs in vivo. C57Bl/6J mice were injected four times intraperitoneally with the LRRK2 kinase inhibitor MLi-2 (10 mg/kg), the CK1 inhibitor PF-670462 (50 mg/kg), or DMSO as a negative control over a period of 30 h. Brain (a), lung (b), and kidney (c) extracts were taken 2 h after the last injection and analyzed with immunoblotting using MJFF-2 anti-LRRK2 antibody, anti-LRRK2 P-S935, or anti-α-tubulin or anti-vinculin for equal loading. Representative blots are shown. Graphs show the quantification of blots representing the ratio of total LRRK2 signal over housekeeping protein signal or the ratio of phosphorylation at S935 over total LRRK2 signal. Error bars indicate S.E.M. with N ≥ 3. Statistical significance was tested using column statistics with Bonferroni correction. Triple asterisks indicate p < 0.001, double asterisks indicate p < 0.01, asterisk indicates p < 0.05