Pharmacology of cis and trans-LAB-Glu.
a) Puffing cis-LAB-Glu to a hippocampal CA1 pyramidal neuron at different concentrations (0.05, 0.1, 0.25 mM) for 100 ms. b) Left: Puffing trans-LAB-Glu (0.047 mM, i.e. 0.1 mM LAB-Glu at the violet PSS) onto a CA1 pyramidal neuron for different durations (5, 10, 25, 50 ms). Right: Quantification of the relationship between trans-LAB-Glu evoked currents and puffing durations. c) The currents evoked by 1 sec puffing of trans-LAB-Glu (control) were blocked by DL-AP5 (40 μM), but not CNQX (10 μM), implying LAB-Glu activates NMDA receptors. All experiments were repeated in 3 cells.