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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: ACS Chem Neurosci. 2019 Feb 15;10(5):2481–2488. doi: 10.1021/acschemneuro.8b00734

Figure 6.

Figure 6.

Photoswitching of LAB-QA inside CA1 neurons.

a) Cartoon illustrating how LAB-QA binds to the inner vestibule of potassium channels in the trans configuration. b) Currents from a CA1 pyramidal neuron responding to voltage steps (−60 to +40 mV) with LAB-QA (0.5 mM) in patch recording pipette with violet and green light (left). Bidirectional modulation of voltage-gated ion channels with LAB-QA. The electrophysiological protocol consisted of depolarizing voltage steps from −60 mV to +40 mV for 200 ms at a frequency of 1 Hz. This depolarization activated voltage-gated potassium channels were reversible blocked by violet and green light (right). c) Multiple rounds photoswitching of LAB-QA inside a CA1 neuron. This is representative of traces obtained from 3 cells. Voltage steps as in b).