(a) RT-qPCR analysis of sirtuin transcripts in WT, p53−/−,
G4/p53 +/+ and G4/p53 −/− liver tissue demonstrates that
mitochondrial sirtuins (Sirt3, 4 & 5) are repressed in G4/p53 +/+ mice and
p53 deficiency in G4 mice rescues their expression (9 mice per group analyzed);
(b) Western blot analysis using total liver tissue or isolated liver
mitochondria shows elevated sirtuin protein expression in G4/p53
−/− mice compared to G4/p53 +/+ mice (9 mice per group were
analyzed); (c) Combined IP-western blot analysis of liver tissues derived from
WT, p53 −/−, G4/p53 +/+ and G4/p53 −/− mice
demonstrates decreased acetylation levels of PGC-1α, FOXO1, SOD2, CPS1 in
G4/p53 −/− compared to G4/p53 +/+ mice (6 mice per group were
analyzed); (d) Analysis of acetylation levels of mitochondrial proteins from WT,
p53 −/−, G4/p53 +/+, and G4/p53 −/− mice shows that
G4/p53 −/− have decreased acetylation compared to G4/p53 +/+ mice
(6 mice per group were analyzed). Results are quantified by densitometry and
expressed as mean ± s.e.m.; t-test was used to determine statistical
significance with p <0.05 considered as significant, as indicated by
(*).