Figure 3.

Aryl hydrocarbon receptor (AhR)-dependent induction of interleukin-33 (IL-33) expression in THP-1 macrophages. THP-1 macrophages were pretreated with an AhR antagonist, CH223191 (10 μM), 20 min before treatment with 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) (10 nM) or kynurenine (KYN) (50 μM). Six hours after the AhR agonist treatment, total RNA was prepared from the cells, followed by real-time PCR measurement of the (A) CYP1A1 and (B) IL-33 variant 5 mRNA levels. The values represent the mean ± SE (n = 4). Data were analyzed using a one-way analysis of variance (ANOVA), followed by Dunnett's test or a t test. **p < .01 versus the control group. ##p < .01 versus the No Add group.