Figure 5.
Normalized gene expression of (A) angiotensin II receptor type 1, (B) angiotensin II receptor type 2, and (C) angiotensin converting enzyme (ACE) mRNA, as determined by real-time qPCR, in BBB coculture astrocytes treated with mouse plasma (1:20 dilution in media) on the endothelial (apical) layer from C57Bl/6 mice exposed to either filtered air (FA) or mixed vehicle exhaust (MVE: 100 μg/m3 PM) for 6 h/d for 30 days on either a low-fat (LF) or high-fat (HF) diet. D, Secreted ACE levels were also assessed in the media of the same astrocyte cultures by ELISA. Data represented as mean ± SEM from n = 6. *p < .05 compared with LF+FA. †p < .05 compared with HF+FA. ‡p < .05 compared with LF+MVE.