Figure 6.
Quantification of secreted (A) interleukin-6 (IL-6) and (B) transforming growth factor-beta (TGF-β) levels, as assessed by ELISA in astrocyte media from BBB coculture astrocytes treated with mouse plasma (1:20 dilution in media) on the endothelial (apical) layer from C57Bl/6 mice exposed to either filtered air (FA) or mixed vehicle exhaust (MVE: 100 μg/m3 PM) for 6 h/d for 30 days on either a low-fat (LF) or high-fat (HF) diet. Normalized gene expression of astrocyte (C) aryl hydrocarbon receptor and (D) glutathione peroxidase mRNA, as determined by real-time qPCR, from the same conditions listed above for panels A and B. Data represented as mean ± SEM from n = 6. *p < .05 compared with LF+FA. †p < .05 compared with HF+FA. ‡p < .05 compared with LF+MVE.