TABLE 3.
Primers used in this study
| Primer | Sequence (5→3) | Description |
|---|---|---|
| kopicA1A2A3-UF | AGCTTGATATCGAATTCCTGCAGCTGATTTTGCCAAGATCTATC | To construct plasmid pJQ-ΔpicA1A2A3 |
| kopicA1A2A3-UR | TCTAGAACTAGTGGATCCTTCCATCGGCAACATGCACTGC | |
| kopicA1A2A3-DF | GGATCCACTAGTTCTAGACCGTCGTGGCCGAGTTCAATCC | |
| kopicA1A2A3-DR | AGGGAACAAAAGCTGGAGCTCGGCAGGCAACATGAACAGCAC | |
| kopicB1B2B3B4-UF | AGCTTGATATCGAATTCCTGCAGACTCGGAGCGCGACTGCTCAC | To construct plasmid pJQ-ΔpicB1B2B3B4 |
| kopicB1B2B3B4-UR | TCTAGAACTAGTGGATCCGTATAAGTCCGTGGTTTGCATC | |
| kopicB1B2B3B4-DF | GGATCCACTAGTTCTAGAGTTTCGGAAATTTTTATCCAGT | |
| kopicB1B2B3B4-DR | AGGGAACAAAAGCTGGAGCTCTCTGCGCGTAAGGCACCAGTTC | |
| picA1A2A3-F | CAGGAATTCGATATCAAGCTTTACGACAAGTCTCAGGAGCTGTGG | To construct plasmid pBBR-picA1A2A3 |
| picA1A2A3-R | GGTACCGGGCCCCCCCTCGAGGTACCATTGCACTTCCAGCCAGG | |
| picA1A2-F | CAGGAATTCGATATCAAGCTTTACGACAAGTCTCAGGAGCTGTGG | To construct plasmid pBBR-picA1A2 |
| picA1A2-R | GGTACCGGGCCCCCCCTCGAGGTCGCGGCTGGTGGCCAACATGC | |
| picA2A3-F | CAGGAATTCGATATCAAGCTTATCGCCCAATACGGAGTTTGG | To construct plasmid pBBR-picA2A3 |
| picA2A3-R | GGTACCGGGCCCCCCCTCGAGGTACCATTGCACTTCCAGCCAGG | |
| picB1B2B3B4-F | GGTACCGGGCCCCCCCTCGAGGTTGCGGCCGTTGCCGATTTCAG | To construct plasmid pBBR-picB1B2B3B4 |
| picB1B2B3B4-R | CAGGAATTCGATATCAAGCTTGGAATCAGAAGACCTTCTGACTC | |
| picB1B2B3-F | GGTACCGGGCCCCCCCTCGAGGTTGCGGCCGTTGCCGATTTCAG | To construct plasmid pBBR-picB1B2B3 |
| picB1B2B3-R | CAGGAATTCGATATCAAGCTTACGATCCAACGTGGCACAGTAC | |
| picB2B3B4-F | GGTACCGGGCCCCCCCTCGAGGTAGAAACTGGCCCTGCACAAACTGG | To construct plasmid pBBR-picB2B3B4 |
| picB2B3B4-R | CAGGAATTCGATATCAAGCTTGGAATCAGAAGACCTTCTGACTC | |
| picEDF-F | CAGGAATTCGATATCAAGCTTCATCGGCAGCAATATTCTGATCACC | To construct plasmid pBBR-picEDF |
| picEDF-R | GGTACCGGGCCCCCCCTCGAGGTCACATCGTTCTTCTCAAATAC | |
| picEDFG-F1 | CAGGAATTCGATATCAAGCTTCATCGGCAGCAATATTCTGATCACC | To construct plasmid pBBR-picEDFG |
| picEDFG-R1 | CCAAGCATCGTCGCACATTTCCCACATCGTTCTTCTCAAATAC | |
| picEDFG-F2 | GGAAATGTGCGACGATGCTTGG | |
| picEDFG-R2 | GGTACCGGGCCCCCCCTCGAGGTGGCAGGGCTGTGTACTGCTAAG | |
| expPicD-F | CTTTAAGAAGGAGATATACATATGAGCACATTTTTGTATGGC | To construct plasmid pET-PicD |
| expPicD-R | GTGGTGGTGGTGGTGGTGCTCGAGCACCAAGCGCTGGCCCAG | |
| RT-16S F | CGCGGTAATACGTAGGGTG | To amplify fragment 16S shown in Fig. 1 |
| RT-16S R | AACTTCACGCGTTAGCTGCG | |
| RT-1 F | TCGTCGGGGTTGATACGACG | To amplify fragment 1 shown in Fig. 1 |
| RT-1 R | GGCCGACATCCTGGGTCCAGTG | |
| RT-2 F | GTACGTCGTGCTGTCGCAGACT | To amplify fragment 2 shown in Fig. 1 |
| RT-2 R | ATAGAACGGCACATCCAGCTT | |
| RT-3 F | GGCAGTAACTTGGGGTTTTGTGG | To amplify fragment 3 shown in Fig. 1 |
| RT-3 R | AGAAGGCGCGCATATCCTCAG | |
| RT-4 F | AGATTGCCGCTCAGTCCATGG | To amplify fragment 4 shown in Fig. 1 |
| RT-4 R | TCACGAATCGCAGGGAATTC | |
| RT-5 F | GCAACGGCACGTGAGCAG | To amplify fragment 5 shown in Fig. 1 |
| RT-5 R | CAACCGCTGGTAACCGCAC | |
| RT-6 F | ATACCGGGAACACCAGATCGTTCG | To amplify fragment 6 in Fig. 1 |
| RT-6 R | ATTGAGGCCGAGGGTAATTTCATGC | |
| RT-7 F | GCATACTTGTCCTCGGACTTGG | To amplify fragment 7 in Fig. 1 |
| RT-7 R | ACCAGTGGTCTTACAGCCTGAAGG | |
| RT-8 F | ATCCTGCCCGACAATGGCG | To amplify fragment 8 in Fig. 1 |
| RT-8 R | CACTGTCATTGAAGCGAATG | |
| RT-9 F | AGGCCTGGCTAAAGTCAGCC | To amplify fragment 9 in Fig. 1 |
| RT-9 R | AAATTTCCGAAACCGATGC | |
| RT-10 F | CGCCGCCCGTGCTGACCGC | To amplify fragment 10 in Fig. 1 |
| RT-10 R | GCAGGCGGCCTTCATTGC | |
| RT-11 F | CCCACGCTCAGATTGAAGA | To amplify fragment 11 in Fig. 1 |
| RT-11 R | ACATCCACAGACTCAATAAAC | |
| RT-12 F | GAACGGCTTTACGACGTTTG | To amplify fragment 12 in Fig. 1 |
| RT-12 R | TCCAACGTGGCATTGCCCTTGAAGG | |
| RT-13 F | CCGCATGAGCGTGATGGC | To amplify fragment 13 in Fig. 1 |
| RT-13 R | TCGTGGTTCAGGCGCTTGAC |