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. 2019 Jul 11;2019:6902906. doi: 10.1155/2019/6902906

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Copyright © 2019 Can Cui et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FOXO3 knockdown in chicken granulosa cells. (a) The relative mRNA expression level of FOXO3 after the knockdown. (b) Western blotting analysis of FOXO3 protein levels after FOXO3 knockdown. (c) The relative mRNA expression levels of the apoptosis-related genes after FOXO3 knockdown. (d) Western blot analysis of cleaved PARP-1 and caspase 3 in control and FOXO3 knockdown cells. β-Actin served as a reference. Data are expressed as means ± SEM (n = 3 independent cell cultures). ∗P<0.05; ∗∗P<0.01.