Method summary	UV thermal melting (indirect)
Requirements	Spectrometer equipped with cell holder containing Peltier heat pumps connected to an external temperature controller. Quartz cuvettes with PTFE stoppers (commonly used).
Results	Thermal melting of phase transitions/molar enthalpy/association constants of metallodrug–nucleic acid interactions.
Advantages	Simple experimental setup. High sensitivity and reproducibility. Suitable for comparing/ranking binding affinity (SAR). Small sample size.
Limitation	Metallodrugs stability/optical transparency at elevated temperatures. Binding information generally provided at non-physiological temperatures (>37 °C).
Complementary and advanced techniques	NMR and viscosity (Sections 2.2 and 2.4) and optical techniques including circular dichroism spectroscopy, indirect fluorometric assays and topoisomerase I-mediated DNA relaxation (Sections 3.1.2, 3.2.1 and 4.2). Advanced complementary techniques include isothermal titration calorimetry (ITC) and qPCR.