Method summary	Circular dichroism (indirect)
Requirements	Circular dichroism spectrometer with continuous nitrogen flow, quartz cuvettes required.
Results	Conformational changes to nucleic acid structure upon metallodrug binding.
Advantages	Excellent sensitivity and reproducibility. Provides structural information related to specific binding interactions. Small sample size.
Limitation	Caution must be taken with chiral metallodrugs. Experimental conditions must carefully maintain DNA structure. Limited solvent choice (UV activity).
Complementary and advanced techniques	Direct binding analysis by UV-vis and fluorescence spectroscopy, DNA unwinding experiments (4.2), and viscosity (2.4).
	Advanced techniques include linear dichroism along with X-ray crystallography, NMR, UV thermal melting (Sections 2.1, 2.2 and 3.1.1) and molecular dynamics.