. 2019 Jul 18;10:1695. doi: 10.3389/fimmu.2019.01695

Table 1.

Allele-specific transcript quantification by NGS.

	Sample type	Depth of coverage^a	Balance^b	Counts (G allele)	Frequency (G allele) %
THYROID
102	gDNA	29548x	0.49	15,054	50.95
	cDNA	9305x	0.49	4,926	52.94
218	gDNA	25800x	0.49	13,283	51.48
	cDNA	5006x	0.49	2,746	54.85
355	gDNA	15027x	0.49	7,771	51.71
	cDNA	9200x	0.50	4,940	53.70
381	gDNA	25044x	0.49	12,633	50.44
	cDNA	23579x	0.49	12,361	52.42
409	gDNA	25049x	0.50	12,726	50.80
	cDNA	14221x	0.49	7,211	50.71
430	gDNA	29863x	0.49	15,038	50.36
	cDNA	1693x	0.49	764	45.13
452	gDNA	32564x	0.49	16,484	50.62
	cDNA	1738x	0.50	877	50.46
92	gDNA	20911x	0.49	10,717	51.25
	cDNA	13439x	0.49	6,916	51.46
THYMUS
101	gDNA	28664	0.49	14,453	50.42
	cDNA	6933	0.49	3,960	57.12
117	gDNA	35762	0.49	18,282	51.12
	cDNA	5912	0.49	3,764	63.67
119	gDNA	24245	0.49	12,165	50.18
	cDNA	4570	0.49	2,663	58.27
126	gDNA	28552	0.50	14,280	50.01
	cDNA	4510	0.49	2,481	55.01
145	gDNA	33290	0.50	16,932	50.86
	cDNA	17575	0.49	9,996	56.88
159	gDNA	25178	0.50	12,664	50.30
	cDNA	6616	0.49	4,299	64.98
16	gDNA	22447	0.49	11,221	49.99
	cDNA	13429	0.49	8,649	64.41
171	gDNA	30800	0.49	15,640	50.78
	cDNA	4138	0.49	2,438	58.92
188	gDNA	29066	0.49	14,720	50.64
	cDNA	342	0.48	185	54.09
195	gDNA	31457	0.49	16,255	51.67
	cDNA	3656	0.50	2,235	61.13
197	gDNA	31543	0.49	16,169	51.26
	cDNA	8058	0.49	4,385	54.42
225	gDNA	26017	0.49	13,152	50.55
	cDNA	18406	0.49	11,164	60.65
229	gDNA	31049	0.50	15,955	51.39
	cDNA	7373	0.50	4,392	59.57
235	gDNA	31949	0.49	16,325	51.10
	cDNA	14662	0.49	8,516	58.08
263	gDNA	33124	0.49	16,852	50.88
	cDNA	16402	0.49	9,478	57.79
28	gDNA	27355	0.49	13,931	50.93
	cDNA	6143	0.49	3,593	58.49
38	gDNA	4047	0.49	2,110	52.14
	cDNA	6701	0.50	4,253	63.47
4	gDNA	25273	0.49	12,879	50.96
	cDNA	16769	0.49	9,802	58.45
72	gDNA	31112	0.49	15,957	51.29
	cDNA	7426	0.49	4,277	57.59

Results of cDNA samples are indicated in bold.

The number of unique reads (i.e., sequences) which interrogate the position of rs179247.

Balance between reads from the forward or reverse primer. A value of 0.5 indicates that half of the reads come from each primer (i.e., there is no strand bias).