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(A) Section of an original image after manual thresholding. (B) White pixels were classified as ‘border’ by the random forest segmentation model. (C) Initial cluster. (D) Distance transformation of the non-border pixels. The following thresholding was conducted with parameter τ = 0.3. (E) Results of applying the watershed algorithm to the thresholded image yields two final clusters. (F) GAC snake reconstruction of the two final clusters.
